MSH2 and MLH1 immunodetection and the prognosis of colon cancer

Citation
J. Perrin et al., MSH2 and MLH1 immunodetection and the prognosis of colon cancer, INT J ONCOL, 19(5), 2001, pp. 891-895
Citations number
20
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
10196439 → ACNP
Volume
19
Issue
5
Year of publication
2001
Pages
891 - 895
Database
ISI
SICI code
1019-6439(200111)19:5<891:MAMIAT>2.0.ZU;2-5
Abstract
MSH2 and MLH1 are proteins involved in DNA reparation. They are mutated in some forms of colon cancer, i.e. hereditary non-polyposis. carcinomas and a subset of sporadic carcinomas. We have studied the expression of MSH2 and MLH1 in a retrospective series of 225 colorectal carcinomas by immunohistoc hemistry. The results were compared to molecular tests of microsatellite in stability using amplification by PCR of BAT-25 and BAT-26 repeated sequence s and to histoclinical data. Positivity of both proteins was never associat ed with MSI phenotype. MSH2 and/or MLH1 negative tumors were frequently tum ors of the proximal colon; in this subpopulation or proximal tumors, MSH2 a nd/or MLH1 negativity was associated with a longer disease-free survival.