It has been suggested that lactate concentrations may provide a guide to an
optimal training intensity. However, lactate concentrations established du
ring incremental exercise in the laboratory are not always indicative of wh
at is occurring during constant-load exercise at the same intensity. Ideall
y, lactate concentrations should be measured during a training session and
immediately reported to the athlete to ensure that the athlete is working a
t the desired intensity. The purpose of this investigation was, therefore,
to determine the reliability and validity of a compact, portable lactate an
alyser (ACCUSPORT; Boeringer Mannheim, Castle Hill, Australia). A total of
224 capillary blood samples were taken from athletes who took part in routi
ne laboratory testing. Seventy-three of these capillary blood samples were
analysed in duplicate with the Accusport for determination of intraclass, s
ingle-trial reliability. Day-to-day intraclass reliability of the Accusport
was assessed by analyzing known concentrations of aqueous lactate solution
s on seven consecutive days. The validity of the Accusport analyser was ass
essed by comparing the 224 capillary blood lactate concentrations determine
d on the Accusport with the lactate concentration obtained using a MICRO ST
AT LM3 (Analox Instruments Ltd., London, UK). In addition, lactate paramete
rs derived from the lactate concentrations obtained with the two analysers
were compared. The Accusport showed high single-trial intraclass reliabilit
y (R = 0.992; Standard Error of Measurement [SEM] = 0.3 mmol x I-1; n = 73)
and high day-to-day intraclass reliability (R = 0.993; SEM = 0.4 mmol x I-
1; n = 42). Despite a strong correlation between blood lactate concentratio
ns obtained on the two analysers (r = 0.96; n = 224) the limits of agreemen
t were + 1.9 to - 2.2 mmol x I-1. Although the mean values for power output
, HR and lactate concentration associated with the lactate parameters were
not significantly different when determined on the Accusport or Micro Stat,
some individuals did record large differences between analysis methods. In
summary, the results of this investigation have shown that lactate concent
rations can be reliably determined within a single trial and from day-to-da
y using the Accusport analyser. However, for some athletes, it is not valid
to compare lactate concentrations or lactate parameters determined on the
Accusport with those determined using the Micro Stat LM3 lactate analyser.