Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis

Authors
Blank, LM Koebmann, BJ Michelsen, O Nielsen, LK Jensen, PR
Citation
Lm. Blank et al., Hemin reconstitutes proton extrusion in an H+-ATPase-negative mutant of Lactococcus lactis, J BACT, 183(22), 2001, pp. 6707-6709
Citations number
18
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
22
Year of publication
2001
Pages
6707 - 6709
Database
ISI
SICI code
0021-9193(200111)183:22<6707:HRPEIA>2.0.ZU;2-W
Abstract
H+-ATPase is considered essential for growth of Lactococcus lactis. However , media containing hemin restored the aerobic growth of an H+-ATPase-negati ve mutant, suggesting that hemin complements proton extrusion. We show that inverted membrane vesicles prepared from hemin-grown L. lactis cells are c apable of coupling NADH oxidation to proton translocation.