Ca. Santos et al., Optimisation of the biological treatment of hypersaline wastewater from Dunaliella salina carotenogenesis, J CHEM TECH, 76(11), 2001, pp. 1147-1153
The reutilisation of Dunaliella salina carotenogenesis medium, after microa
lgal biomass separation by centrifugation, was assessed. The wastewater had
an NaCl concentration between 174 g dm(-3) and 254 g dm(-3) and an average
total organic matter concentration of 1540 mg dm(-3) ash-free dry weight,
of which 41% (w/v) was glycerol. The biological treatment was established a
t laboratory scale and batch operations used halophilic bacteria from the w
astewater itself. The wastewater was supplemented with NH4+, PO43-, K+ and
Mg2+ ions to enhance growth. The effect of each ion added per se was initia
lly investigated and a response surface methodology (RSM) used to identify
the optimal conditions for maximisation of glycerol removal from the wastew
ater, which was considered to be the main objective. Addition of NH4+ ions
alone achieved 79% glycerol removal compared with only 59% in the absence o
f supplement, after 8 days incubation. The combined addition of ions ([NaCl
] =214g dm [Mg2+] = 114 mg dm(-3), [K+] = 131 mg dm(-3), [NH4+] = 113 mg dm
(-3), [PO43-] = 40 mg dm(-3)) increased glycerol removal from the wastewate
r such that, after 2 days incubation, no residual glycerol was apparent in
cultures. These ion combinations enabled the halophilic bacteria to efficie
ntly remove glycerol from the wastewater and consequently reduce organic ma
tter. This treated wastewater should be appropriate for reutilisation as a
carotenogenesis medium for beta -carotene production from D salina. (C) 200
1 Society of Chemical Industry.