IL-2-driven natural killer cell generation: Role of anti-H-2(b) monoclonalantibodies and stromal cells in controlling quantitative and repertoire changes
M. Agostini et al., IL-2-driven natural killer cell generation: Role of anti-H-2(b) monoclonalantibodies and stromal cells in controlling quantitative and repertoire changes, J CHEMOTHER, 13(5), 2001, pp. 527-534
To investigate the role of major histocompatibility complex class I and bon
e marrow stromal cells on in vitro differentiation of natural killer cells,
a CD44(low)/(-)CD2(-) population was isolated from mouse bone marrow. This
NK-1.1(-)CD3(-)LFA-3(+)B220(+) population, when stimulated with IL-2 and c
o-cultured with supportive syngeneic stromal cells, generated populations o
f NK-1.1(+)Ly49A(+)Ly49C/I(+)CD3(-) mature natural killer cells. The effect
of anti-H-2(b) monoclonal antibodies (mAbs) on this phenomenon was assayed
. Pre-adhesion of anti-H-2(b) mAbs to the stromal cells did not exert any e
ffect, whereas when the same mAbs were pre-adhered to progenitors, there wa
s a inhibition of natural killer cell generation that was maximum when the
mAbs were added directly to cultures. In addition, the anti-H-2(b) mAbs did
not inhibit the IL-2-induced proliferation of mature natural killer cells.
Allogeneic but not H-2(b)-deficient stromal cells decreased the expression
of Ly-49C/I but not Ly49A, thus suggesting that stromal cell haplotypes qu
alitatively influence the expression of Ly49s repertoire.