Simultaneous quantitation of nucleoside HIV-1 reverse transcriptase inhibitors by short-end injection capillary electrochromatography on a beta-cyclodextrin-bonded silica stationary phase

As part of our on-going study of the analysis and quantitation of anti-HIV nucleosides, a capillary electrochromatography (CEQ method has been develop ed for the simultaneous quantitation of nucleoside HIV reverse transcriptas e inhibitors (NRTIs), i.e. zidovudine (AZT), lamivudine (3TC), didanosine ( ddA) and its administrated form (ddI), stavudine (d4T) and hivid (ddC). CEC on chiral stationary phase has mainly been dedicated to the separation of enantiomers. However, this paper explores an original application of a beta -cyclodextrin-bonded silica packed column, taking advantage of the interna l hydrophobicity of the polysaccharide to separate the NRTIs. The influence of several parameters pH buffer, ionic strength, acetonitrile content, tem perature and voltage) has been investigated using the short-end injection t echnique to achieve baseline separation in a short-time analysis before qua ntitation. (C) 2001 Elsevier Science B.V. All rights reserved.