The effects of androgens and estrogens on preadipocyte proliferation in human adipose tissue: Influence of gender and site

Citation
La. Anderson et al., The effects of androgens and estrogens on preadipocyte proliferation in human adipose tissue: Influence of gender and site, J CLIN END, 86(10), 2001, pp. 5045-5051
Citations number
39
Categorie Soggetti
Endocrynology, Metabolism & Nutrition","Endocrinology, Nutrition & Metabolism
Journal title
JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
ISSN journal
0021972X → ACNP
Volume
86
Issue
10
Year of publication
2001
Pages
5045 - 5051
Database
ISI
SICI code
0021-972X(200110)86:10<5045:TEOAAE>2.0.ZU;2-Z
Abstract
The gender-specific differences in body fat distribution suggest that sex s teroids play an important role in regulating body fat distribution. Sex ste roids may regulate adipose tissue mass by altering adipocyte number and siz e. The effects of various sex steroids on in vitro proliferation of preadip ocytes from both se and omental fat depots was investigated in men and wome n. Abdominal se and omental preadipocytes from men (n = 14) and women (8 pr emenopausal and 7 postmenopausal) were cultured in the presence of 17 beta -E2 (10(-7)-10(-9) M), estrone (10(-7)-10(-9) M), or dehydrotestosterone (D HT) (10(-7)-10(-9) M), and the rate of proliferation was measured over time (1-96 h) by DNA accumulation assays (micromoles per mug) and [H-3]thymidin e incorporation (disintegrations per min). In se preadipocytes the rate of proliferation was increased between 24-48 h with E2 (10(-7) M) in both men (P = 0.028) and women (P = 0.017). Subcutaneous preadipocytes from women we re more responsive to E2 in stimulating proliferation than those from men ( women vs. men, DNA assay, 24 h, P = 0.014). In omental preadipocytes the in crease in the rate of proliferation occurred at 24 h with E2 (10(-7) M) in women (P = 0.034) and at 48 h in men (P = 0.031). Gender appeared to influe nce the rate of proliferation by E2 in omental preadipocytes, with maximal stimulation of proliferation at 48 h in preadipocytes from women treated wi th E2 (10(-7) M, p = 0.007) compared with 72 h in preadipocyte cells from m en (P = 0.048), as shown by DNA assay. Both estrone and the androgen DHT ha d no significant gender- or site-specific effect on the rate of proliferati on at any time point. All DNA content data were further validated by thymid ine incorporation analysis. In summary, E2 stimulates the rate of prolifera tion of preadipocytes in a dose-dependent manner, with significant gender- and site. specific differences. Neither estrone nor DRT affected adipocyte mass through proliferation of preadipocytes in this study. In conclusion, E 2 may act as an important local factor influencing the proliferation of pre adipocytes that may affect fat cell number in a depot- and gender-specific pattern in human abdominal se and omental adipose tissue.