Association of jaagsiekte sheep retrovirus with pulmonary carcinoma in sardinian moufflon (Ovis musimon)

Bronchiolo-alveolar carcinoma has been described in man and in several anim al species, including cattle, dogs, opossums, goats and sheep. In sheep, a bronchiolo-alveolar carcinoma, known as ovine pulmonary carcinoma (OPC), is caused by jaagsiekte sheep retrovirus JSRN, an exogenous type D retrovirus . In the mid-1980s, a severe outbreak of a disease resembling OPC was descr ibed in captive Sardinian moufflon (Ovis musimon). In the present study, th e use of polymerase chain reaction (PCR) amplification of nucleic acids ext racted from archival material established that JSRV was associated with OPC in affected moufflon. JSRV was detected in the lungs and mediastinal lymph nodes. Immunohistochemical and in-situ PCR demonstrated that in the lungs, JSRV proviral. DNA was localized in transformed and untransformed type II pneumocytes and in the alveolar macrophages. In the mediastinal lymph nodes , JSRV DNA was mainly located in the cortical follicles and paracortex. The se data suggest that JSRV is the cause of OPC in Sardinian moufflon, as it is in Sardinian sheep. (C) 2001 Harcourt Publishers Ltd.