Neurotoxic effects of fifth-generation dentin adhesives on rat sciatic nerve

This study evaluated the effect of the fifth-generation dentin adhesives, S ingle Bond (SB), Excite (EX), Prime & Bond NT (PB), and Optibond Solo (OS) on nerve conduction. Isolated rat sciatic nerves were placed between two su ction electrodes in a bath containing tyrode solution. The bonding agents w ere brought into contact with the nerves and the evoked compound action pot entials (cAPs) were recorded before and after contact with the materials. S B, EX, and PB caused total inhibition of the cAPs, with PB being the fastes t (73 +/-5.8 min). All cAPs except one in the PB group were irreversibly in hibited in the SB, EX, and PB groups. As for OS the reduction in cAP was no t 50% after an application time of 200 min. Recovery of the cAPs in this gr oup was recorded in an average time of 35 min.