Primers for amplification of mt SSU rDNA, and a phylogenetic study of Botryosphaeria and associated anamorphic fungi

Two PCR primers (MSU1 and MSU7) were designed to amplify part of the mt SSU rDNA gene from filamentous ascomycetes. The MSU1-MSU7 and MSU1-NMS2 primer pairs were tested with 32 isolates of 23 species in 10 genera. Minimum evo lution, parsimony, and likelihood methods were used for the phylogenetic an alyses of rn SSU rDNA sequences from Botryosphaeria species and related tax a, These analyses indicated that: Botryosphaeria is not monophyletic; the s eparation of Botryosphaeria from Guignardia is supported; except for B. vac cinii, other Botryosphaeria species and related taxa form a monophyletic gr oup with strong jackknife support; Diplodia pinea f. sp. cupressi is differ ent from RAPD marker groups A and B of Sphaeropsis sapinea; the anamorph ge nera Sphaeropsis and Diplodia are not indicative of close relationships amo ng different species; and the name B. stevensii may have been applied to mo re than one species. Botryosphaeria needs additional study and substantial taxonomic revision.