A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR

Authors
Subbotin, SA Peng, DL Moens, M
Citation
Sa. Subbotin et al., A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR, NEMATOLOGY, 3, 2001, pp. 365-371
Citations number
29
Categorie Soggetti
Animal Sciences
Journal title
NEMATOLOGY
ISSN journal
13885545 → ACNP
Volume
3
Year of publication
2001
Part
4
Pages
365 - 371
Database
ISI
SICI code
1388-5545(2001)3:<365:ARMFTI>2.0.ZU;2-#
Abstract
A method for rapid identification of juveniles and cysts of the soybean cys t nematode based on PCR with species specific primers is described. The PCR assay was tested on 53 populations originating from China, Russia, USA and Brazil. A single cyst or second stage juvenile of Heterodera glycines alon e or in a mixture with other soil inhabiting nematodes was detectable.