Multiple sclerosis. Chlamydia hypothesis put to the test

Recently, an association between multiple sclerosis and Chlamydia pneumonia e infection has been suggested. Because standardized PCR protocols are lack ing, a series of studies could not clarify whether C. pneumoniae is present in brain tissue and CSF of MS patients. Therefore, other studies focused o n the humoral immune response against C. pneumoniae: 24% of MS patients, bu t only 5% of the control patients showed intrathecally produced antibodies against C.pneumoniae. If an infection with C.pneumoniae was involved in the pathogenesis of MS, one would expect that, in analogy to other infections of the CNS,the oligoclonal bands in the CSF of MS patients would recognize the responsible agent. However the results we obtained by affinity-mediated immunoblots showed that the oligoclonal bands in the CSF of MS patients ar e not directed against Chlamydia antigen. In contrast to this, we found tha t the immunoglobulins in the CSF of neuroborreliosis patients reacted stron gly against Borrelia antigen in the affinity-mediated immunoblots. In light of these results we assume that the intrathecal immunoglobulin production against C. pneumoniae is part of a polyspecific immune response. Thus, it i s not likely that C.pneumoniae is causally linked to the pathogenesis of mu ltiple sclerosis.