Agrobacterium tumefaciens AKE10-mediated transformation of an Asian pea pear, Pyrus betulaefolia Bunge: host specificity of bacterial strains

Citation
J. Kaneyoshi et al., Agrobacterium tumefaciens AKE10-mediated transformation of an Asian pea pear, Pyrus betulaefolia Bunge: host specificity of bacterial strains, PL CELL REP, 20(7), 2001, pp. 622-628
Citations number
24
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
07217714 → ACNP
Volume
20
Issue
7
Year of publication
2001
Pages
622 - 628
Database
ISI
SICI code
0721-7714(200110)20:7<622:ATATOA>2.0.ZU;2-J
Abstract
The Asian pea pear, Pyrus betulaefolia Bunge, is tolerant to several disord ers in the fruit bodies caused by high humidity and dryness and is hence wi dely used as a rootstock for many pear plants suitable for food sources. We have now successfully transformed P. betulaefolia Bunge by an Agrobacteriu m-mediated gene transfer system. Among several wild-type A. tumefaciens str ains examined, only AKE10 induced shoot-forming tumors at a high frequency on excised cotyledons of P. betulaefolia Bunge cultured on phytohormone-fre e medium. Both the nptII (kanamycin resistance) and GUS (beta -glucuronidas e) genes were introduced into the cotyledons by infection with AKE10 harbor ing a binary vector, and regenerated plants were obtained. Southern hybridi zation and polymerase chain reaction analyses and histochemical GUS assay i ndicated that morphologically normal transformed plants faithfully containe d genes from the vector but not from wild-type oncogenic T-DNA. However, mo rphologically abnormal plants additionally possessed the 6b gene (AK-6b) of AKE10. These results show that non-disarmed A. tumefaciens is adequate to transfer genes to the Asian pea pear, P. betulaefolia Bunge.