Rr. Stange et al., Constituents from the periderm and outer cortex of Ipomoea batatas with antifungal activity against Rhizopus stolonifer, POSTH BIOL, 23(2), 2001, pp. 85-92
Rhiopus stolonifer invades sweetpotato roots through injuries and infected
roots are rapidly consumed by a soft rot. However, not all injuries are equ
ally susceptible to infection; shallow injuries (1-2 mm. deep) are less pro
ne to infection than deeper injuries ( > 5 nim deep). The presence of antif
ungal compounds in external tissues may partially explain the resistance of
shallow injuries to infection. To test this hypothesis, we developed a qua
ntitative bioassay for measuring the growth of R. stolonifer utilizing the
vital stain 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (M
TT). Acetone extracts of the fresh interior flesh of four cultivars had no
antifungal activity, but extracts of the exterior 2 mm of the root were inh
ibitory. We used the R. stolonifer bioassay to guide the purification of th
e active components. Two active fractions were isolated. One active fractio
n contained predominately caffeic acid, but this compound was determined no
t to be the most biologically active component. The second active fraction
contained 3,5-dicaffeoylquinic acid (3,5-DCQA), and this compound was found
to be active, with an EC50 of 2.2 g 1(-1). The presence of antifungal comp
ounds in the external tissues helps explain why shallow injuries are resist
ant to infection. Additionally, we demonstrated that interior flesh tissues
accumulate antifungal compounds when elicited and incubated under curing c
onditions (30 degreesC and 90-95% RH) for 24 h. (C) 2001 Elsevier Science B
.V. All rights reserved.