Visualizing recycling synaptic vesicles in hippocampal neurons by FM 1-43 photoconversion

Exo-endocytotic turnover of synaptic vesicles (SVs) at synapses between hip pocampal neurons in culture was examined by electron microscopy (EM). We ca rried out photoconversion (PC) of the fluorescent endocytotic marker FM 1-4 3 by using 3,3'-diaminobenzidine to convert the dye signal into an electron -dense product. Electron-dense products were located almost exclusively in SVs, whose densities were bimodally distributed in two sharply demarcated p opulations, PC-positive (PC+) and PC-negative (PC-). The median densities o f these populations did not vary with the proportion of vesicles stained wi thin a presynaptic terminal (bouton). The proportion of PC+ SVs remained co nstant across consecutive thin sections of single boutons, but varied great ly from one bouton to another, indicating marked heterogeneity in exo-endoc ytotic activity. Our experiments indicated that only a minority of SVs were stained in most boutons after stimuli known to cause complete turnover of the functional vesicular pool. A direct spatial correlation was found betwe en FM 1-43 fluorescent spots seen with light microscopy and PC+ boutons by EM. The correlation was clearer in isolated boutons than in clusters of bou tons. Photoconversion in combination with FM dyes allows clarification of i mportant aspects of vesicular traffic in central nervous system nerve termi nals.