CRYSTAL-STRUCTURE OF A TRAPPED PHOSPHOENZYME DURING A CATALYTIC REACTION

Authors
LEE YH OLSON TW OGATA CM LEVITT DG BANASZAK LJ LANGE AJ
Citation
Yh. Lee et al., CRYSTAL-STRUCTURE OF A TRAPPED PHOSPHOENZYME DURING A CATALYTIC REACTION, Nature structural biology, 4(8), 1997, pp. 615-618
Citations number
24
Categorie Soggetti
Biology,"Cell Biology
Journal title
Nature structural biology → ACNP
ISSN journal
10728368
Volume
4
Issue
8
Year of publication
1997
Pages
615 - 618
Database
ISI
SICI code
1072-8368(1997)4:8<615:COATPD>2.0.ZU;2-A
Abstract
The crystal structure of the fructose-2,6-bisphosphatase domain trappe d during the reaction reveal a phosphorylated His 258, and a water mol ecule immobilized by the product, fructose-6-phosphate. The geometry s uggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the cataly tic water.