TRAPPING AND VISUALIZATION OF A COVALENT ENZYME-PHOSPHATE INTERMEDIATE

Authors
MURPHY JE STEC B MA L KANTROWITZ ER
Citation
Je. Murphy et al., TRAPPING AND VISUALIZATION OF A COVALENT ENZYME-PHOSPHATE INTERMEDIATE, Nature structural biology, 4(8), 1997, pp. 618-622
Citations number
30
Categorie Soggetti
Biology,"Cell Biology
Journal title
Nature structural biology → ACNP
ISSN journal
10728368
Volume
4
Issue
8
Year of publication
1997
Pages
618 - 622
Database
ISI
SICI code
1072-8368(1997)4:8<618:TAVOAC>2.0.ZU;2-7
Abstract
Using a mutant version of E. coli alkaline phosphatase, we succeeded i n trapping and determining the structure of the phospho-enzyme interme diate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.