In order to better understand the process of cell entry for non-envelo
ped viruses, we have solved the crystal structures of five poliovirus
mutants which can infect cells expressing mutant poliovirus receptors.
Four of these structures have been solved from frozen crystals using
cryocrystallographic data collection methods, The mutations have a ran
ge of structural consequences, from small local perturbations to signi
ficant loop rearrangements. All of the mutant viruses are more labile
to conversion to an apparent cell entry intermediate, suggesting that
these mutant viruses could compensate for the suboptimal receptors by
lowering the thermal energy required to undergo the receptor-mediated
conformational change.