A. Frasoldati et al., Computer-assisted cell morphometry and ploidy analysis in the assessment of thyroid follicular neoplasms, THYROID, 11(10), 2001, pp. 941-946
Conventional cytology cannot discriminate between benign and malignant foll
icular neoplasms. Our study evaluated the diagnostic role of computer-assis
ted image analysis in the presurgical assessment of thyroid follicular neop
lasms. Fifty-eight patients (14 males, 44 females, age range, 45-75 years)
who underwent surgery for cytologic diagnosis of thyroid follicular neoplas
m were studied. All patients were first evaluated on clinical grounds and a
ssigned a high/low suspicion of malignancy on the basis of gender, age, and
nodule size. Cell image analysis was subsequently performed using a Cytome
trica BYK Gulden microscope image processor on Feulgen-stained thyroid cyto
logic smears. A different population of 50 benign and 50 malignant, histolo
gically evaluated nodules was studied in order to establish image analysis
criteria suggestive of thyroid malignancy. Ploidy histogram, proliferation
index (PI), nuclear area coefficient of variation (NACV), and anisocariosis
ratio (AR) were studied. Thyroid cancer was diagnosed in 16 of 58 follicul
ar neoplasms. Only 7 of these lesions were clinically suspicious (43.7%), w
hereas 14 of 16 (87.5%) malignant tumors were identified by image analysis.
Positive and negative predictive values of image analysis versus clinical
evaluation were 46.6% versus 30.4% and 92.8% versus 74.3%, respectively. Th
e distribution of ploidy pattern was different in benign versus malignant f
ollicular neoplasms (chi (2) 8.25, p = 0.016), malignant lesions showing an
increased frequency of heteroclonal aneuploid DNA content (37.5% vs. 7.1%)
. Increased PI (mean +/- standard deviation (SD) = 11.3 +/- 5.7 vs. 7.1 +/-
6.1; p < 0.01) and NACV (mean +/- SD = 25.28 +/- 1.89 vs. 20.14 +/- 0.91;
p < 0.01) levels were also observed in malignant follicular neoplasms. In c
onclusion, computer-assisted image analysis may profitably support clinical
evaluation in the assessment of thyroid follicular neoplasms.