S. Muralidhara et al., Acute, subacute, and subchronic oral toxicity studies of 1,1-dichloroethane in rats: Application to risk evaluation, TOXICOL SCI, 64(1), 2001, pp. 135-145
1,1-Dichloroethane (DCE) is a solvent that is often found as a contaminant
of drinking water and a pollutant at hazardous waste sites. Information on
its short- and long-term toxicity is so limited that the U.S. EPA and ATSDR
have not established oral reference doses or minimal risk levels for the v
olatile organic chemical (VOC). The acute oral LD50 in male Sprague-Dawley
(S-D) rats was estimated in the present study to be 8.2 g/kg of body weight
(bw). Deaths appeared to be due to CNS depression and respiratory failure.
In an acute/subacute experiment, male S-D rats were given 0, 1, 2, 4, or 8
g DCE/kg in corn oil by gavage for 1, 5, or 10 consecutive days. The anima
ls were housed in metabolism cages for collection of urine and sacrificed f
or blood and tissue sampling 24 h after their last dose. There were decreas
es in body weight gain and relative liver weight at all dosage levels, as w
ell as increased renal nonprotein sulfhydryl levels at 2 and 4 g/kg after 5
and 10 days. Elevated serum enzyme levels, histopathological changes, and
abnormal urinalyses were not manifest. For the subchronic study, adult male
S-D rats were gavaged with 0.5, 1, 2, or 4 g DCE/kg 5 times weekly for up
to 13 weeks. Animals receiving 4 g/kg exhibited pronounced CNS depression,
with more than one-half dying by week 11. The 2-g/kg rats exhibited moderat
e CNS depression. One 2-g/kg rat died during week 6. There were very few ma
nifestations of organ damage in animals that succumbed or in survivors at a
ny dosage level. Decreases in bw gain and transient increases in enzymuria
were noted at 2 and 4 g/kg. Serum enzyme levels and blood urea nitrogen wer
e not elevated, nor were glycosuria or proteinuria present. Chemically indu
ced histological changes were not seen in the liver, kidney, lung, brain, a
drenal, spleen, stomach, epididymis, or testis. Hepatic microsomal cytochro
me P450 experiments revealed that single, high oral doses of DCE did not al
ter total P450 levels, but did induce CYP2E1 levels and activity and inhibi
t CYP1A1 activity. These effects were reversible and regressed with repeate
d DCE exposure. There was no apparent progression of organ damage during th
e 13-week subchronic study, nor appearance of adverse effects not seen in t
he short-term exposures. One g/kg orally (po) was found to be the acute, su
bacute, and subchronic LOAEL for DCE, under the conditions of this investig
ation. In each instance, 0.5 g/kg was the NOAEL.