Trends and progress in the characterization of stationary phases for reversed-phase liquid chromatography

The state-of-the-art and progress in the characterization of reversed-phase liquid chromatographic (RPLC) columns are discussed. Existing column tests are compared with tests under development. The validity of several test pa rameters, e.g. hydrophobicity and silanol activity, is also discussed. Solv ent and eluent properties are treated with respect to retention and selecti vity. Developments to improve the chemical stability of RPLC phases and thu s column lifetime are reviewed. It is shown that substantial progress has b een made in the manufacturing of RPLC columns, enabling their use over a wi de variety of experimental conditions. In addition, general measures to sig nificantly extend their lifespan are discussed. The substantial improvement s in batch-to-batch and column-to-column repeatabilities achieved during th e last two decades are also reviewed. (C) 2001 Elsevier Science B.V. All ri ghts reserved.