Sp. Myoda et Cp. Huang, A microscope system with a dual-band filter for the simultaneous enumeration of Cryptosporidium parvum oocysts and sporozoites, WATER RES, 35(17), 2001, pp. 4231-4236
A dual band filter set was designed to allow, the simultaneous viewing of t
wo fluorochromes (fluorescein isothiocyanate (FITC) and 4',6-diamidino-2-ph
enylindole (DAPI)) so that Cryptosporidium oocysts and sporozoites can be v
iewed together. Simultaneous viewing increases accuracy and decreases exami
nation time as compared to current Environmental Protection Agency (EPA) Me
thod 1623 for the detection of Cryptosporidium and Giardia in water by filt
ration/IMS/FA (Immunomagnetic Separation/Fluorescent Antibodies). Additiona
lly, a microscope equipped with a programmable, motorized stage, CCD camera
, and display monitor was used to facilitate well slide evaluation in a qui
ck and precise fashion ensuring complete coverage without overlap and elimi
nating the optical strain associated with counting hundreds of images throu
gh an eyepiece. (C) 2001 Elsevier Science Ltd. All rights reserved.