A microscope system with a dual-band filter for the simultaneous enumeration of Cryptosporidium parvum oocysts and sporozoites

Citation
Sp. Myoda et Cp. Huang, A microscope system with a dual-band filter for the simultaneous enumeration of Cryptosporidium parvum oocysts and sporozoites, WATER RES, 35(17), 2001, pp. 4231-4236
Citations number
8
Categorie Soggetti
Environment/Ecology
Journal title
WATER RESEARCH
ISSN journal
00431354 → ACNP
Volume
35
Issue
17
Year of publication
2001
Pages
4231 - 4236
Database
ISI
SICI code
0043-1354(200112)35:17<4231:AMSWAD>2.0.ZU;2-0
Abstract
A dual band filter set was designed to allow, the simultaneous viewing of t wo fluorochromes (fluorescein isothiocyanate (FITC) and 4',6-diamidino-2-ph enylindole (DAPI)) so that Cryptosporidium oocysts and sporozoites can be v iewed together. Simultaneous viewing increases accuracy and decreases exami nation time as compared to current Environmental Protection Agency (EPA) Me thod 1623 for the detection of Cryptosporidium and Giardia in water by filt ration/IMS/FA (Immunomagnetic Separation/Fluorescent Antibodies). Additiona lly, a microscope equipped with a programmable, motorized stage, CCD camera , and display monitor was used to facilitate well slide evaluation in a qui ck and precise fashion ensuring complete coverage without overlap and elimi nating the optical strain associated with counting hundreds of images throu gh an eyepiece. (C) 2001 Elsevier Science Ltd. All rights reserved.