Pathogenic and pathological characteristic of new type gosling viral enteritis first observed in China

AIM To study the purifying method and characteristics of new gosling viral enteritis virus (NGVEV), the etiological agent of new gosling viral enterit is (NGVE) which was first recognized in China, as well as the pathomorpholo gical development in goslings infected artificially with NGVEV. METHODS (1) NGVEV virions were purified by the procedure of treatment with chloroform and ammonium sulfate precipitation, dialysis to remove the sulfa te radical and ammonium ion and separation by gel filtration chromatography , and SDS-PAGE. (2) Forty 2-day-old White Sichuan goslings were orally admi nistered with NGVEV and 24 hr later 2 birds were randomly selected and kill ed at 24hr intervals until death occurred. Specimens(duodenum, ileum, liver , heart, kidney, spleen, lung, proventriculus, pancreas, esophagus, and the intestinal embolus) were taken until all birds in this group died and were sectioned and stained with hemotoxylin and eosin and studied by light micr oscope. RESULTS NGVEV shared the typical characteristics of Adenovirus and which st ructural proteins consisted of 15 polypeptides. Necrosis and sloughing of t he epithelial cells covering the villus tips of the duodenum were first obs erved in goslings 2 days postinfection artificially with NGVEV. With the pr ogress of infection, this lesion rapidly occurred in the epithelium at the base of the villus and with infiltration of the inflammatory cells, the jej unum tended to be involved. With the intensification of mucosa necrosis and inflammatory exudation of the small intestine, fibrinonecrotic enteritis w as further developed and embolus composed of either intestinal contents wra pped by pseudomembrane or of the mixture of fibrous exudate and necrotic in testinal mucosa were observed in the middle-lower part of the small intesti ne. This structure occluded the intestinal tract and made the intestine dil ated in appearance. The intestinal glandular cells underwent degeneration, necrosis and might be found sloughed into the lumen. Hemorrhage-and hyperem ia could be observed on the lung and kidney. Epithelial cells. of the renal tubular underwent degeneration. In some cases, granular degeneration and-f atty degeneration could be found in the liver and in some cases at a later stage of this disease the epithelial cells of trachea and proventriculus mi ght be found sloughed In some cases at an early stage of this disease, card iac hyperemia and hemorrhage could be observed. Esophagus, pancreas and bra in were found normal. Analyses and comparisons between the pathologic lesio ns of NGVE and Gosling Plague (GP) were available in this paper as well. CONCLUSION (1) NGVEV is adenovirus. (2) Pathological characteristic could b e as the data for NGVE diagnosis.