Europium. sensitized chemiluminescense determination of rufloxacin

A novel chemiluminescence (CL) flow system for rufloxacin (RFX) combined fl ow-injection analysis is presented in this paper. It is based on the energy transfer from RFX to europium(III), the intense luminescence instead of th e weak CL produced by cerium(IV)-sulfurous acid-RFX CL system can be observ ed when Eu(III) was added to the system. A narrow and highly intense emissi on band at 617 nm arised from the excited-state Eu(M) was obtained. Under t he optimum experimental conditions, the linear range was 2.0 x 10(-8) to 5. 0 x 10(-7) M and the detection limit was 5.0 x 10(-9) M. The method has bee n successfully applied to determination RFX in dosage form, serum samples a nd urine samples. The recoveries were 95.0-104.9% for serum and urine sampl es. (C) 2001 Elsevier Science B.V. All rights reserved.