A high-performance liquid chromatographic system equipped with on-line reducer, clean-up and concentrator columns for determination of trace levels of nitropolycyclic aromatic hydrocarbons in airborne particulates

A HPLC system with on-line reducer, clean-up and concentrator columns has b een developed for determining nitropoly-cyclic aromatic hydrocarbons (NPAHs ). Maximum efficiency of the reducer column (Pt/Rh-coated alumina) was achi eved after the column was activated with a flow of air/hydrogen at 350 degr eesC for 3 h and when ethanol-acetate buffer was used as the sample carrier solution. After a benzene-ethanol extract from airborne particulates was w ashed with sodium hydroxide, sulfuric acid and water, an aliquot was inject ed into the system. NPAHs were separated from interfering substances by the clean-up column and reduced to their amino derivatives by the reducer colu mn. The derivatives were concentrated on the concentrator column and eluted into the separator column. NPAHs such as 1,3-, 1,6-, 1,8-dinitropyrenes an d 1-nitropyrene were determined chemilumigenically with linear calibration graphs from I x 10(-11) to 1 x 10(-8) and I x 10(-10) to I X 10(-7) M, resp ectively, at an injection volume of 100 mul. These NPAHs in airborne partic ulates were determined by the proposed method. (C) 2001 Elsevier Science B. V. All rights reserved.