Local expression and distribution of a storage protein in the ovary of Hyphantria cunea

Storage protein-1 (HcSP-1) is a major storage protein found in the hemolymp h and fat body of Hyphantria cunea. HcSP-1 has a high methionine (6.0%) and low aromatic amino acid content (8.5%) (Cheon et al., 1998). In this study , the accumulation and expression of HcSP-1 in ovary was investigated using biochemical and immunocytochemical methods. HcSP-1 was detected in the ova ries in 6-day-old pupae and accumulated toward the end of pupal life, when HcSP-1 transcripts were detectable by Northern blot analysis and RT-PCR. In situ hybridization showed that the HcSP-1 mRNA was located in the nurse ce lls and follicular epithelial cells, but not in the oocyte. Though most of the HcSP-1 that is incorporated in the yolk bodies of the oocyte is probabl y sequestered from the surrounding hemolymph, HcSP-1 is an important yolk p rotein contributing to early yolk body formation before the development of patency by the follicular epithelium. (C) 2001 Wiley-Liss, Inc.