Matrix metalloproteinase-2 and-9 in bile as a marker of liver metastasis in colorectal cancer

Matrix metallproteinases (MMP)-2 and -9 are associated with cancer invasion and metastasis. MMP-2 and MMP-9 activities have never been assayed in bile . In the present study we investigated whether MMP-2 and -9 activities in t he bile could be a marker for evaluation of liver metastasis in colorectal cancer. Fifty-three patients underwent colorectal resection for histologica lly verified adenocarcinoma. Twenty-six patients had colorectal cancer with out liver metastasis and 27 patients had metastatic liver tumor. Six patien ts were studied as carcinoma-free control. MMP-2 and MMP-9 activities were assayed in bile using gelatin zymography and quantitated. Active MMP-2 acti vity of colorectal cancer with liver metastasis group (24.1 +/- 2.5 pixel c ount) was significantly higher than that of colorectal cancer without liver metastasis group (11.4 +/- 1.3 pixel count) (P < 0.001) or of control grou p (6.4 +/- 1.0 pixel count) (P < 0.0010). Active MMP-9 was not detected in bile. ProMMP-9 activity of colorectal cancer with liver metastasis group (5 30.3 +/- 127.5 pixel count) was significantly higher than that of colorecta l cancer without liver metastasis group (213.9 +/- 33.2 pixel count) (P = 0 .008). This is the first report showing that the levels of active MMP-2 and proMMP-9 in bile were significantly higher in liver metastasis of colorect al cancer than in metastasis-free colorectal cancer. The results suggest th at activities of active MMP-2 and proMMP-9 in the bile may be useful marker s for predicting liver metastasis in colorectal cancer. (C) 2001 Academic P ress.