Identification of gene clusters differentially expressed during the cellular injury responses (CIR) to cisplatin

The goal of this study was to identify changes in mRNA levels in tumour cel ls after a toxic exposure to cisplatin (IC99 dose). Using suppression-subtr active hybridization (SSH) 2 cDNA libraries were created, an UP library (20 2 cDNA fragments) and a DOWN library (153 cDNA fragments). Using reversed N orthern hybridization 16 and 30 fragments were truly differentially express ed in the UP and DOWN libraries, respectively. Most prominent in the UP lib rary were the mitochondrial and injury response clusters and in the DOWN li brary the cytoskeletal. protein synthesis and signalling clusters. These di stinct clusters potentially represent an expression profile of the cisplati n-induced cellular injury response. (C) 2001 Cancer Research Campaign.