Development and characterisation of monoclonal antibodies raised against aviral haemorrhagic septicaemia virus (VHSV) isolate which failed to be identified by a commercial test kit

Monoclonal antibodies (mAb) were generated against viral haemorrhagic septi caernia virus (VHSV) strain Wi, which was isolated from rainbow trout in Ge rmany in 1992. Isolates of this VHSV variant did not react in the indirect immunofluorescent assay (IIF) with a commercially available anti-VHSV mAb. In HF nine of the resulting 24 mAbs against VHSV isolate Wi recognized 30 V HSV strains and isolates representing the whole genotypic and phenotypic ra nge known so far. Another group consisting of three mAbs detected these vir uses too with the difference that they failed to react with marine virus is olates of pacific origin. In Western blot analysis, 18 of the resulting mAb s reacted with the glycoprotein G, one mAb with the matrix protein M of VHS V, and three mAbs failed to react. Only one mAb was able to neutralize some virus strains.