Regional mapping of suppressor loci for anchorage independence and tumorigenicity on human chromosome 9

By micro cell-mediated chromosome transfer to the malignant Syrian hamster cell line BHK-191-5C, we previously identified two suppressor functions on human chromosome 9 (HSA9), one for anchorage independence and another for t umorigenicity. However, the precise chromosomal locations of these suppress or functions were not determined. The present study was undertaken to defin e the regional location of these suppressor loci using a panel of microcell hybrids containing structurally altered HSA9 with different deleted region s in the BHK-191-5C background. DNA derived from the cell hybrids was analy zed by PCR for verification of the presence of HSA9 genetic material by amp lifying 62 microsatellite markers and 13 genes, covering the entire length of HSA9. Our deletion mapping data on anchorage independent and tumorigenic hybrids suggest that the suppressor function for anchorage independence is located in the region between 9q32 to 9qter. The suppressor for tumorigeni city may be located in one of three deleted regions on HSA9, the first one between the markers D9S162 and D9S1870, the second one between the markers D9S1868 and TIGRA002I21, and the third one between the markers D9S59 and D9 S155. (C) 2001 Elsevier Science Inc. All rights reserved.