Expression of the cdc25B gene as a prognosis marker in non-small cell lungcancer

There is an evidence to suggest that cdc25B phosphatase is an oncogenic. We hypothesized that cdc25B gene may be expressed in tumors of patients with non-small cell lung cancer (NSCLC) and affect their clinical outcome. Expre ssion of cdc25B messenger RNA was evaluated by reverse transcription polyme rase chain reaction in 55 non-small cell lung carcinomas and adjacent histo logical normal lung samples using LightCycler. The data was analyzed in ref erence to clinicopathological data and survival data. There was no differen ce of cdc25B expression level between the NSCLC tissue and normal lung tiss ue. There was no relationship between cdc25B gene expression and age, gende r, N or T-status and clinical stage. However, the NSCLC patients with high cdc25B expression had significantly poor survival than the patients with lo w cdc25B expression (P = 0.0173). Thus we suggest that cdc25B may predict p oor survival. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.