Effectiveness of two fluoridation measures on erosion progression in humanenamel and dentine in vitro

The aim of the present study was to evaluate the effect of fluoride on the progression of erosive demineralisation in human enamel and dentine using a cyclic de- and remineralisation model in vitro. The mineral content expres sed in micrometres was determined daily by longitudinal microradiography (L MR) and presented as cumulative mineral loss over 5 days. For erosive demin eralisation, all samples were immersed in 0.05 M citric acid (pH 2.3) for 6 x 10 min/day and stored in a remineralisation solution. Fluoridation measu res were performed as follows: group 1: control, no fluoridation; group 2: toothpaste fluoridation 3 x 5 min/daily (NaF, 0.15% F-); group 3: toothpast e fluoridation as group 2 and additionally application of a fluoride mouthr inse (Olaflur/SnF2, 0.025%F-) 3 x 5 min/daily and on days 1 and 3 gel fluor idation (Olaflur/NaF; 1.25% F-) for 1 x 5 min. After the first experimental day, no significant differences were found between the groups. However, af ter 5 days the erosive mineral loss values for enamel were 147.5 +/- 18.7 m um in the control group, 128.1 +/- 15.0 mum in group 2 (p less than or equa l to0.05) and 116.1 +/- 12.4 mum in group 3 (p less than or equal to0.001). In dentine, the respective values were 136.7 +/- 16.4, 111.8 +/- 26.9 (p l ess than or equal to0.001) and 60.3 +/- 17.8 (P less than or equal to0.001) . The intensive fluoridation significantly reduced erosion progression in e namel but had a more pronounced effect on dentine. The results suggest that subjects with erosive lesions should use an intensive fluoridation measure . Copyright (C) 2001 S. Karger AG, Basel.