R. Landry et al., Characterization of syndecan-4 expression in 3T3-F442A mouse adipocytes: Link between syndecan-4 induction and cell proliferation, CELL GROWTH, 12(10), 2001, pp. 497-504
Heparan sulfate proteoglycans are found on the surface of most cells. Synde
can-4 is a widely expressed transmembrane heparan sulfate proteoglycan. Usi
ng quantitative RNase protection assays and immunoblotting, syndecan-4 expr
ession was characterized in 3T3-F442-A mouse adipoblasts. These cells exhib
it dramatic changes in their biological and morphological characteristics d
uring differentiation to adipocytes. During this process, the levels of syn
decan-4 protein and mi expression changed dramatically. They peaked at the
time when quiescent cells reentered the cell cycle before differentiation.
Serum depletion-repletion also replicated the syndecan-4 mRNA induction whe
n the cells were released back into proliferation, and a cycloheximide trea
tment abolished the peak of induction. In addition, inhibiting syndecan-4 i
nduction with antisense oligonucleotides Inhibited the proliferation of 3T3
-F442A cells. In the terminally differentiated adipocytes characterized by
the loss of proliferation capability, the serum Inducibility of syndecan-4
is repressed, emphasizing the link between syndecan-4 induction in 3T3-F442
A cells and cell proliferation.