K. Iba et al., Phase-independent inhibition by retinoic acid of mineralization correlatedwith loss of tetranectin expression in a human osteoblastic cell line, CELL STRUCT, 26(4), 2001, pp. 227-233
We have recently reported that retinoic acid inhibits dexamethasone-induced
alkaline phosphatase activity and mineralization in human osteoblastic cel
l line SV-HFO. In this study, we show that this inhibitory effect on alkali
ne phosphatase activity depends on the stage of cell differentiation; howev
er, expression of tetranectin, which is a recently reported bone matrix pro
tein, was completely inhibited by treatment with retinoic acid, irrespectiv
e of the stage of cell differentiation. Similarly, mineral deposit formatio
n in SV-HFO cells was phase-independently inhibited by retinoic acid. To ou
r knowledge, this is the first report that retinoic acid downregulates the
tetranectin expression in human osteoblastic cells independent of the stage
of cell differentiation, and is correlated with inhibition of mineralizati
on.