Comparison of anti-PR3 capture and anti-PR3 direct ELISA for detection of antineutrophil cytoplasmic antibodies (ANCA) in long-term clinical follow-up of PR3-ANCA-associated vasculitis patients
O. Arranz et al., Comparison of anti-PR3 capture and anti-PR3 direct ELISA for detection of antineutrophil cytoplasmic antibodies (ANCA) in long-term clinical follow-up of PR3-ANCA-associated vasculitis patients, CLIN NEPHR, 56(4), 2001, pp. 295-301
A total of 118 sera from 11 patients with anti-neutrophil cytoplasmic antib
odies against proteinase-3- (PR3-ANCA) associated vasculitis were retrospec
tively screened by anti-PR3 capture and anti-PR3 direct ELISA tests. We stu
died the relationship between capture and direct ELISA scores and the clini
cal activity of PR3-ANCA-associated vasculitis patients during follow-up. W
e also studied the ability of the anti-PR3 capture ELISA to detect positive
values of PR3-ANCA in clinical vasculitis relapses. Only capture ELISA pre
sented a significant relationship (p < 0.05) with clinical activity of PR3-
ANCA-associated vasculitis patients over time. Capture ELISA appears to be
a reliable method for detecting clinical relapses in this group of patients
. Our results indicate that the new capture ELISA test is more effective th
an direct ELISA in the follow-up of patients with PR3-ANCA-associated vascu
litis and in the detection of relapses.