Oxidative stress markers in pre-uremic patients

Aim: The present study was designed to investigate a complex of oxidative s tress (OS) markers in patients with chronic renal failure (CRF) and to stud y the relationship between different OS markers and degree of renal failure . The following indices of OS were measured in plasma: oxidized glutathione (GSSG), reduced glutathione (GSH), total glutathione (TGSH), glutathione r edox ratio (GSSG/GSH) and resistance of lipoprotein fraction to oxidation ( lag phase of LPF). Baseline diene conjugation level of lipoprotein fraction (BDC-LPF), total antioxidative activity (TAA), diene conjugates (DC), lipi d hydroperoxides (LOOH) and thiobarbituric acid-reactive substances (TBARS) were measured in serum. All markers in plasma and ser-um were measured bot h in patients with CRF and in healthy controls. Subjects and methods: Blood samples were obtained from 38 patients with CRF and from 61 healthy contro ls. Routine biochemical analyses were performed by using commercially avail able kits. Results: Levels of DC, BDC-LPF, LOOH, GSSG and GSSG/GSH ratio we re significantly increased and lag phase of LPF was significantly shortened in patients with CRF compared with healthy controls. Serum creatinine and urea levels correlated significantly with GSSG level and GSSG/GSH in patien ts with CRF. A significant inverse correlation was found between glutathion e redox ratio and lag phase of LPF and between GSSG level and BDC-LPF. Conc lusions: The findings suggest that renal patients are in a state of oxidati ve stress compared with healthy controls. The most informative indices to e valuate the degree of OS in CRT were: GSSG level, GSSG/GSH status, lag phas e of LPF and BDC-LPF.