The zebrafish buttonhead-like factor Bts1 is an early regulator of pax2.1 expression during mid-hindbrain development

Little is known about the factors that control the specification of the mid -hindbrain domain (MHD) within the vertebrate embryonic neural plate. Becau se the head-trunk junction of the Drosophila embryo and the MHD have patter ning similarities, we have searched for vertebrate genes related to the Dro sophila head gap gene buttonhead (btd), which in the fly specifies the head -trunk junction. We report here the identification of a zebrafish gene whic h, like btd, encodes a zinc-finger transcriptional activator of the Sp-1 fa mily (hence its name, bts1 for btd/Sp-related-1) and shows a restricted exp ression in the head. During zebrafish gastrulation, bts1 is transcribed in the posterior epiblast including the presumptive MHD, and precedes in this area the expression of other MHD markers such as her5, pax2.1 and wnt1. Ect opic expression of bts1 combined to knock-down experiments demonstrate that Bts1 is both necessary and sufficient for the induction of pax2.1 within t he anterior neural plate, but is not involved in regulating her5, wnt1 or f gf8 expression. Our results confirm that early MHD development involves sev eral genetic cascades that independently lead to the induction of MHD marke rs, and identify Bts1 as a crucial upstream component of the pathway select ively leading to pax2.1 induction. In addition, they imply that flies and v ertebrates, to control the development of a boundary embryonic region, have probably co-opted a similar strategy: the restriction to this territory of the expression of a Btd/Sp-like factor.