High-performance genetic analysis using microfabricated capillary array electrophoresis microplates

This review focuses on some recent advances in realizing microfabricated ca pillary array electrophoresis (mu CAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the h igh-speed collection of sequencing and genotyping data from radially format ted mu CAE devices. The concomitant development of a convenient energy-tran sfer cassette labeling chemistry allows sensitive multicolor labeling of an y DNA genotyping or sequencing analyte. High-performance hereditary haemoch romatosis and short tandem repeat genotyping assays are demonstrated on the se devices along with rapid mitochondrial DNA sequence polymorphism analysi s. Progress in supporting technology such as robotic fluid dispensing and b atched data analysis is also presented. The ultimate goal is to develop a p arallel analysis platform capable of integrated sample preparation and auto mated electrophoretic analysis with a throughput 10-100 times that of curre nt technology.