Chromatography dynamics for the separation of the large molecules compounds

In the work the chromatographic separation of a mixture of two large molecu le compounds (analytes) - methyl esters of. deoxycholic (D) and cholic (C) acids was performed. Ternary mixture of solvents containing: hexane, ethyl acetate and methanol as eluent modifier was used as a mobile phase. The inf luence of the eluent composition on the separation process was analysed, th e dependency of isotherm parameters and mass transport kinetics on the elue nt composition was determined. For the analysis the transport - dispersive model consisting of the mass balances equations of the analytes and the mod ifier lumped with isotherm equations was used.