Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens

Authors
Collins, E Glennon, M Hanley, S Murray, AM Cormican, M Smith, T Maher, M
Citation
E. Collins et al., Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens, J CLIN MICR, 39(11), 2001, pp. 4163-4165
Citations number
12
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
11
Year of publication
2001
Pages
4163 - 4165
Database
ISI
SICI code
0095-1137(200111)39:11<4163:EOAPPC>2.0.ZU;2-I
Abstract
DNA was extracted from 50 human stool specimens using the QIAamp DNA stool minikit. PCR amplification was followed by post-PCR hybridization to DNA pr obes specific for the Campylobacter genus, Campylobacter jejuni, and Campyl obacter coli in a colorimetric membrane assay. Thirty-two of 38 culture-pos itive specimens were PCR/DNA probe positive for C jejuni. The assay is rapi d and simple and can be applied to stool specimens for the detection of Cam pylobacter.