To determine the contribution of haemoglobin (Hb) to the hypoxia-tolerance
of Daphnia magna, we exposed Hb-poor and Hb-rich individuals (2.4-2.8mm lon
g) to a stepwise decrease in ambient oxygen partial pressure (PO2amb) over
a period of 51min from normoxia (20.56kPa) to anoxia (<0.27kPa) and looked
for differences in their physiological performance. The haem-based concentr
ations of Hb in the haemolymph were 49 mu moll(-1) in Hb-poor and 337 mu mo
ll(-1) in Hb-rich animals, respectively. The experimental apparatus made si
multaneous measurement of appendage beating rate (fA), NADH fluorescence in
tensity (I-NADH) of the appendage muscles, heart rate (fh) and in vivo Hb o
xygen-saturation possible. In response to progressive, moderate hypoxia, bo
th groups showed pronounced tachycardia and a slight decrease in fA. The fA
and fH of Hb-rich animals were generally 4-6 % lower than those of Hb-poor
animals. In addition, Hb-rich animals showed a significant decrease in the
P-O2amb at which the Hb in the heart region was half-saturated and a strik
ing reduction in the critical P-O2amb Of appendage-related variables. In Hb
-poor animals, the INADH signal indicated that the oxygen supply to the lim
b muscle tissue started to become impeded at a critical P-O2amb of 4.75 kPa
, although the high level of fA was largely maintained until 1.77 kPa. The
obvious discrepancy between these two critical P-O2amb values suggested an
anaerobic supplementation of energy provision in the range 4.75-1.77 kPa. T
he fact that I-NADH of Hb-rich animals did not rise until PO,amb fell below
1.32 kPa strongly suggests that the extra Hb available to Hb-rich animals
ensured an adequate oxygen supply to the limb muscle tissue in the P-O2amb
range 4.75-1.32 kPa. This finding illustrates the physiological benefit of
Hb in enabling the animal to sustain its aerobic metabolism as the energeti
cally most efficient mode of fuel utilization under conditions of reduced o
xygen availability.