L. Salas-cortes et al., Expression of the human SRY protein during development in normal male gonadal and sex-reversed tissues, J EXP ZOOL, 290(6), 2001, pp. 607-615
Sex determination in mammals is controlled by the SRY gene located on the Y
chromosome. It encodes a protein containing a DNA-binding and DNA-bending
domain. In spite of recent advances in the identification of the mechanisms
that regulate male sex determination in mammals, the expression profile of
the SRY protein in normal and sex-reversed human tissues is not well estab
lished. In order to localize the SRY protein and determine its cellular dis
tribution and expression at different stages of development, we prepared mo
noclonal antibodies (mAb) against the recombinant SRY protein. One of these
antibodies, LSRY1.1, recognizes a protein of 27 kDa in total lysates of He
La SRYB3, a human cell line transfected with the SRY gene under the control
of the SV40 promoter. Immunocytochemical analysis in the cell lines shows
nuclear localization of the SRY protein. We have studied SRY protein expres
sion in human tissues at different stage of fetal development until adult l
ife and have demonstrated that the SRY protein is located in the nuclei of
somatic cells and germ cells in the genital ridge during testis development
. After testis determination, it can be detected until the adult stage in b
oth germ cells and Sertoli cells. The presence of the SRY protein was also
analyzed in biopsies of gonadal tissues of sex-reversal patients such as SR
Y-positive 46,XX males or SRY-positive 46,XX true hermaphrodites. SRY prote
in is detected in the nuclei of Sertoli cells of the testis and in the nucl
ei of granulosa cells in the ovotestis in these patients and in the nuclei
of germ cells of both tissue types. These results suggest a common cellular
origin for both Sertoli cells and granulosa cells. J. Exp. Zool. 290:607-6
15, 2001. (C) 2001 Wiley-Liss, Inc.