Improving vaccine potency through intercellular spreading and enhanced MHCclass I presentation of antigen

Citation
Cf. Hung et al., Improving vaccine potency through intercellular spreading and enhanced MHCclass I presentation of antigen, J IMMUNOL, 166(9), 2001, pp. 5733-5740
Citations number
31
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
166
Issue
9
Year of publication
2001
Pages
5733 - 5740
Database
ISI
SICI code
0022-1767(20010501)166:9<5733:IVPTIS>2.0.ZU;2-Q
Abstract
The potency of naked DNA vaccines is limited by their inability to amplify and spread in vivo. VP22, a HSV-1 protein, has demonstrated the remarkable property of intercellular transport and may thus provide a unique approach for enhancing vaccine potency. Therefore, we created a novel fusion of VP22 with a model Ag, human papillomavirus type 16 E7, in a DNA vaccine that ge nerated enhanced spreading and MHC class I presentation of Ag. These proper ties led to a dramatic increase in the number of E7-specific CD8(+) T cell precursors in vaccinated mice (around 50-fold) and converted a less effecti ve DNA vaccine into one with significant potency against E7-expressing tumo rs. In comparison, nonspreading VP22(1-267) mutants failed to enhance vacci ne potency. Our data indicated that the potency of DNA vaccines may be dram atically improved through intercellular spreading and enhanced MHC class I presentation of Ag.