Adsorption dynamics of bovine serum albumin (BSA) onto binary interpenetrating polymer networks (IPNs) of poly(2-hydroxyethyl methacrylate) (PHEMA)

Authors
Bajpai, AK Shrivastava, M
Citation
Ak. Bajpai et M. Shrivastava, Adsorption dynamics of bovine serum albumin (BSA) onto binary interpenetrating polymer networks (IPNs) of poly(2-hydroxyethyl methacrylate) (PHEMA), J MACR S PU, 38(11), 2001, pp. 1123-1139
Citations number
37
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
ISSN journal
10601325 → ACNP
Volume
38
Issue
11
Year of publication
2001
Pages
1123 - 1139
Database
ISI
SICI code
1060-1325(2001)38:11<1123:ADOBSA>2.0.ZU;2-L
Abstract
A binary hydrophilic interpenetrating polymer network (IPN) comprising of p olyethylene glycol (PEG) and poly(2-hydroxyethyl methacrylate) (PHEMA) was prepared and the adsorption of bovine serum albumin (BSA) was carried out o nto the surfaces of the IPNs. The adsorbed amounts of BSA were investigated as a function of the composition of the hydrogel, pH and ionic strength of the protein solution. The adsorption of protein was also studied kinetical ly and various parameters such as the rate constants for adsorption, diffus ion and penetration rate constants were evaluated. For the assessment of th e anti thrombogenic property of the IPNs surfaces, blood-clot formation tes ts were performed.