The photoreceptor cell-specific nuclear receptor is an autoantigen of paraneoplastic retinopathy

Objectives: To report a novel antibody associated with paraneoplastic retin opathy and to characterize the retinal autoantigen. Methods: Immunohistochemistry of rat and human tissues was used to identify antiretinal antibodies. Serologic screening of a bovine retinal cDNA expre ssion library was performed to clone the target antigen. Results: A 72-year-old woman presented with a 6-month history of progressiv e visual loss, bilateral central scotomas, light flashes, and night blindne ss. Visual acuity was 20/40 OD and 20/30 OS. There was generalized loss of retinal pigment and narrow arterioles; discs were normal in appearance. The electroretinogram showed no response. Chest computed tomograph scan demons trated a right lung mass: biopsy revealed poorly differentiated carcinoma. The patients' serum contained antibodies that immunolabeled nuclei of cells of the outer-and to a lesser extent, the inner-nuclear layer of the adult rat retina. No reactivity was identified with nonretinal adult human or rat tissues. Reactivity was seen in the developing rat embryo. Serologic scree ning of a bovine retinal library resulted in the isolation of three overlap ping clones, encoding a protein highly homologous to the human photorecepto r cell-specific nuclear receptor gene product. Conclusions: The target antigen of an antibody associated with paraneoplast ic retinopathy is the photoreceptor cell-specific nuclear receptor, a membe r of a conserved family of nuclear receptors involved in photoreceptor cell development or maintenance.