Short echo time (TE) in vivo PRESS H-1 MR spectra (2 T, TE = 35 ms) of norm
al brain were fitted in the frequency domain using the second derivative me
thod. In this approach, local maxima and hidden peaks are found as local mi
nima of spectrum second derivative. The Lorentzian robust minimisation proc
edure (referred to as maximum likelihood or m-estimate fitting) using Leven
burg-Marquardt non-linear fitting engine was applied. Spectral lines were a
pproximated under the assumption of the mixed Lorentzian/Gaussian lineshape
s. The same procedure was applied to 18 proton spectra. The number of peaks
found within the range of 0.74/4.2 parts per million (ppm) was 52 +/- 3 an
d their positions were almost the same. The fitted lines were assigned on t
he basis of the J-pattern recalculated for the field strength of 2 T and by
comparing the chemical shifts with the shifts in the single compound spect
ra. The ratios of main metabolites, such as NAA/Cr, Cho/Cr, Cho/NAA and mI/
Cr, are in accord with those obtained earlier using the software supplied w
ith the MR imager and the absolute concentrations of N-acetylaspartate (NAA
), choline containing compounds (Cho), myoInositol (mI), glucose (Glc) and
glutamate (Glu) obtained from the fit agree with those reported in literatu
re, which confirms the usefulness of the second derivative method in routin
e analyses of H-1 MR brain spectra. (C) 2001 Elsevier Science B.V. All righ
ts reserved.