Effects of cell adhesion molecules on adhesion of chondrocytes, ligament cells and mesenchymal stem cells

Smooth cell seeding and cell adhesion on temporary porous scaffolds are cri tical for tissue engineering. We investigated that the effects of type I co llagen, type H collagen, fibronectin, vitronectin and poly-L-lysine, lamini n, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan and hyaluronic ac id on adhesion of articular chondrocytes, ligament cells and mesenchymal st em cells (MSCs), for well understanding the interaction between adhesion mo lecules and diverse kinds of cells. The cell adhesion molecules were coated on the surfaces of 96-well culture plates. The cells were inoculated and c ultured in the wells for 30 min, and subsequently, the number of adhered ce lls was determined. Type I collagen, type H collagen, fibronectin, vitronec tin and poly-L-lysine promoted significantly more cell adhesion than lamini n, chondroitin-4-sulfate, chondroitin-6-sulfate, aggrecan and hyaluronic ac id. MSCs showed a similar adhesion tendency as ligament cells. These two ki nds of cells adhered significantly more on fibronectin-coated surface than on type I collagen-, type H collagen-, vitronectin- and poly-L-lysine-coate d surfaces, while the effect was decreased for chondrocytes. Fibronectin sh owed to be most potential to promote cell adhesion, and thus would be effec tive to be incorporated into porous scaffolds for tissue engineering of car tilage and ligament. (C) 2001 Elsevier Science B.V. All rights reserved.