TFIIH functions are altered by the P210BCR-ABL oncoprotein produced on thePhiladelphia chromosome

P210BCR-ABL counteracted against the complementary effect of XPB on DNA rep air when ultraviolet (UV)-sensitive 27-1 cells were treated with UV or cisp latin but not with hydrogen peroxide. Wortmannin, an inhibitor of PI3 kinas e did not affect its anti-repair effect. Enhanced recruitment of p44 with T FIIH after cisplatin treatment is inhibited by the expression of P210BCR-AB L in a kinase activity-dependent manner. Although purified TFIIH from P210B CR-ABL expressor and non-expressor showed almost no difference in molar rat io of each component, the in vitro activity of TFIIH was decreased by 5-10% in repair assay but was increased by more than two-fold in transcription a ssay. (C) 2001 Elsevier Science B.V. All rights reserved.