Assembly of microarrays for genome-wide measurement of DNA copy number.

Authors
Snijders, AM Nowak, N Segraves, R Blackwood, S Brown, N Conroy, J Hamilton, G Hindle, AK Huey, B Kimura, K Law, S Myambo, K Palmer, J Ylstra, B Yue, JP Gray, JW Jain, AN Pinkel, D Albertson, DG
Citation
Am. Snijders et al., Assembly of microarrays for genome-wide measurement of DNA copy number., NAT GENET, 29(3), 2001, pp. 263-264
Citations number
7
Categorie Soggetti
Molecular Biology & Genetics
Journal title
NATURE GENETICS
ISSN journal
10614036 → ACNP
Volume
29
Issue
3
Year of publication
2001
Pages
263 - 264
Database
ISI
SICI code
1061-4036(200111)29:3<263:AOMFGM>2.0.ZU;2-6
Abstract
We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise meas urement (s.d. of log(2) ratios=0.05-0.10) in cell lines and clinical materi al, so that we can reliably detect and quantify high-level amplifications a nd single-copy alterations in diploid, polyploid and heterogeneous backgrou nds.