CMX-8933, a peptide fragment of the glycoprotein ependymin, promotes activation of AP-1 transcription factor in mouse neuroblastoma and rat cortical cell cultures
Ve. Shashoua et al., CMX-8933, a peptide fragment of the glycoprotein ependymin, promotes activation of AP-1 transcription factor in mouse neuroblastoma and rat cortical cell cultures, NEUROSCI L, 312(2), 2001, pp. 103-107
An 8-amino acid peptide fragment (CMX-8933) of Ependymin, a glycoprotein co
mponent of the extracellular fluid and cerebrospinal fluid of goldfish brai
n, was synthesized and tested for its capacity to activate AP-1 transcripti
on factor in cell cultures. Dose-response and time-course studies of AP-1's
binding to DNA were carried out in neuroblastoma (NB2a/ dl) and primary ra
t brain cortical cultures using an electrophoretic mobility shift assay (EM
SA). A 13-14-fold increase in AP-1's DNA binding was obtained when NB2a cel
ls were incubated for 4 h with 6-10 mug/ml CMX-8933. Primary rat brain cort
ical cultures were much more sensitive to the effects of CMX-8933 than tran
sformed (NB2a) cultures; here a 26.7 +/- 5.2-fold increase in binding was o
bserved following a 3-h treatment with as little as 10 ng/ml peptide. These
findings are consistent with an activation of this transcription factor, a
characteristic that has been previously correlated with functional aspects
of full-sized neurotrophic factors (nerve growth factor and brain-derived
nerve growth factor) in neuronal differentiation and regeneration. Such dat
a suggest a role for Ependymin in transcriptional control. (C) 2001 Elsevie
r Science Ireland Ltd. All rights reserved.